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Antibodies bound to the blocking peptide now not bind on the epitope around the concentrate on protein. By comparing the staining from the blocked antibody as opposed to the antibody alone, you can see which staining is distinct.
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind specially into the target antibody and block antibody binding. These peptide typically has the epitope regarded by the antibody.
Target Info Cyclin E belongs for the very conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
Overexpression of this gene has long been observed in several tumors, which results in chromosome instability, and so might lead to tumorigenesis. This protein was found to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical part in endorsing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two further splice variants ended up described but in-depth nucleotide sequence info isn't but offered.
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